Functional Characterization of the Obesity-Linked Variant of the β3-Adrenergic Receptor

Adrenergic receptor β3 (ADRβ3) is a member of the rhodopsin-like G protein-coupled family. The binding ligand to ADRβ3 activates adenylate cyclase and increases cAMP in cells. highly expressed white brown adipocytes controls key regulatory pathways lipid metabolism. Trp64Arg (W64R) polymorphism associated with early development type 2 diabetes mellitus, lower resting metabolic rate, abdominal obesity, insulin resistance. It unclear how substitution W64R affects functioning ADRβ3. This study was initiated functionally characterize this obesity-linked variant We evaluated detail expression, subcellular distribution, post-activation behavior WT using single cell quantitative fluorescence microscopy. When HEK 293 cells, shows typical distribution displayed by other GPCRs predominant localization at surface. Unlike adrenergic β2 (ADRβ2), agonist-induced desensitization does not involve loss surface expression. comparable biochemical properties, there no significant impact tryptophan arginine on cellular signaling, indistinguishable from terms behavior.